Home     Project Overview   Group Members    Collaborators   Contact Info

Myelin Group Publications    Tony's Other Publications    Photos   Links    Views and Opinions


Who Did What in The Myelin Group

Past Members

Current Members

Anthony (Tony) HEAPE: Project Leader (e-mail: aheape (at) cc.oulu.fi). Finnish Academy Research Fellow

Put the project together and, like most Project Leaders, pushes the administrative paper around (when he can understand it) and writes innumerable grant applications trying desparately to find adequate funding that only rarely seems to go where it is most needed. Supervision and coordination of the execution of the project, local and international collaborations and student research training. Coordinating the Myelination & Myelin Disease Consortium (MMDC) in an ambitious new project that was initiated at the beginning of 2004 in the context of the Tekes-funded NeoBio Program. When possible, he even gets to the lab bench, where he has participated as follows:

  • Design and construction of the GST-MAGCT fusion protein vectors
  • Expression of the fusion proteins
  • Production and characterization of the anti-MAGCT antibodies
  • Purification of native MAG, and its isoforms, from rat and human PNS and CNS
  • Protein-protein interaction studies
  • S-MAG dimorphism
  • Native MAG degradation

Satu PÄIVÄLÄINEN (2001- ongoing) (e-mail: sspaival (at) cc.oulu.fi)

Joined The Myelin Group in January 2001 as a postgrad, when we got (at last) our first big research grant (Finnish Academy). She did most of the work aiming to characterize the proteolytic activity responsible for human MAG degradation in the CNS. Following a visit to Professor Eduardo Nobile-Orazio (Milan, Italy), she has set up the myelinating DRG/Schwann cell coculture system in our lab, and will be employing this model to analyse cellular and molecular events implicated in the regulation of PNS myelination.
 

Outi LAHTI (2002 - ongoing) (e-mail: olahti (at) mail.student.oulu.fi)

Joined The Myelin Group in May 2002 as a postgrad, when we got a bit more money. She participated in the characterization of the proteolytic activity responsible for human MAG degradation in the CNS, and also started looking for new MAG cytoplasmic domain ligands (Petri did not find them all). Outi  is now adapting the myelinating coculture system to the mouse, so that we can fully benefit from the extraordinary potential of this model to analyse protein functional networks involved in PNS myelination and hereditary myelin disease.

Henrika HONKANEN (2004 - ongoing) (e-mail: hhonkan (at) cc.oulu.fi)

Joined the Myelin Group in September 2004 as a postgrad. She is responsible for the identification and characterization of intracellular MAG ligands and for analyzing the regulation and roles of the interactions.

Back to Top
 

Past Members

Petri KURSULA (1995-2000) (e-mail: pkursula (at) cc.oulu.fi)

Was around right from the beginning of the project (Autumn 1995) until he produced the first Ph.D. thesis of the Myelin Group (including 7 publications) in Spring 2000. A real work-horse who actually did alot of the real important work and came up with some great ideas. When he wasn't helping to look for money to finance his studies, doing some of the less interesting teaching chores in the department, and doing another full-time job that actually provided a salary, he spent an enormous amount of time at the lab bench, where he participated as follows:

  • Expression of the fusion proteins
  • Identification and characterization of the allelic S-MAG dimorphism in the rat
  • Identification and characterization of the S100b-L-MAG interaction
  • Regulation of L-MAG PKA-dependent phosphorylation by S100b
  • Identification and characterization of the zinc-binding property of S-MAG
  • Identification and characterization of the interaction of S-MAG with tubulin
  • Crystallization of GST-S-MAG fusion protein for structural analyses.

After defending his Ph. D. thesis, he moved to Prof. Rik Wierenga's group (Biocenter Oulu & Department of Biochemistry, Oulu University, Finland), where he was responsible for the crystallography lab. As of the beginning of 2003, Petri went SOUTH, to the EMBL in Hamburg, to work as a Post-doc for... some time.

Jussi HALLEEN (1997)

Spent some time in the Myelin Group during his Military/Civil service year, participating in the study of MAG homodimerization.

Gitte TIKKANEN/MERILÄINEN (1997-98)

As part of her pro-graduate research training project, she undertook the successful construction and production of mutated GST/L-MAG fusion proteins employed in various studies undertaken in the Myelin Group. She is currently doing a Ph.D. in the group of Rik Wierenga (Biocenter Oulu and Department of Biochemistry, Oulu University, Finland).

Marko SUOKAS (1999-2000)

Spent a year in the Myelin Group during his Military/Civil service, optimizing the purification and stabilization of native MAG from human brain tissue. He is currently finishing his Ph.D. work (that was interupted by his "Service" year) in the group of Sakari Kellokumpu (Department of Biochemistry, Oulu University, Finland).

Mervi YLIANTTILA (2001-2002)

As part of her pro-graduate research training project, she undertook the construction and production of mutated His-tagged MAG cytoplasmic domain fusion proteins that we hope to employ in various studies undertaken in the Myelin Group. She also significantly improved the expression level of the old L-MAG/GST fusion protein, which, in its original form, was not only very weakly expressed, but also had a nasty tendency to degrade.

Back to Top