Micropropagation of recalcitrant adult Scots pine and the secondary metabolites of some medicinally important plants

Group leader: Prof. Anja Hohtola, Ph. D., e-mail: anja.hohtola(at)oulu.fi

Group members:

• Anna Maria Pirttilä, Ph. D., e-mail: am.mattila(at)oulu.fi
• Laura Jaakola, Ph. D., e-mail: laura.jaakola(at)oulu.fi
• Katja Karppinen, M. Sc., Ph. D. student, e-mail: katja.karppinen(at)oulu.fi
• Terttu Kämäräinen-Karppinen, M. Sc., Ph. D. student, e-mail: terttu.kamarainen(at)oulu.fi
• Elina Virtanen, M. Sc., Ph. D. student, e-mail: elina.virtanen(at)mtt.fi

Department of Biology/Botany, University of Oulu, P.O.B. 3000, FIN-90014 Oulu, Finland

Our topics are:

• Regeneration of Scots pine starting from adult trees: endophytes and defense
• Synthesis of plumbagin and other naphthoquinones in round leaved sundew
• The secondary metabolism of rose root (Rhodiola rosea)
• Biotechnology and secondary metabolism of bilberry and lingonberry
• The secondary metabolism of Saint John's wort (Hypericum perforatum)
• Production of hardy perennials

We are working in co-operation with:

• Prof. Sirpa Kärenlampi, Department of Biochemistry and Biotechnology, University of Kuopio, Finland
• Doc. Riitta Törrönen, Institute of Applied Biotechnology, University of Kuopio
• Prof. Hely Häggman, Department of Biology, University of Oulu, Finland
• Dr. Sampo Mattila, Department of Chemistry, University of Oulu, Finland
• Prof. Eva Cellarova, P. J. Safarik University, Faculty of Science, Department of Experimental Botany and Genetics, Kosice, Slovakia
• Dr. Terezia Salajová, Institute of Plant Genetics, Slovak Academy of Sciences, Nitra, Slovakia
• Prof. Peter Neubauer, Department of Process Engineering, University of Oulu, Finland
• Prof. Matti Uhari, Dept. of Paediatrics, University of Oulu, Finland
• Dr. Zsuzsanna György, Dept. of Genetics and Plant Breeding, Corvinus University of Budabest, Hungary

Recent publications:

• Koskimäki, J.J., Hokkanen, J., Jaakola, L., Tolonen A., Mattila S., Pirttilä, A.M. & Hohtola, A.(2009) Flavonoid biosynthesis and degradation play role in early defense responses of bilberry (Vaccinium myrtillus L.) against biotic stress. - European Journal of Plant Pathology 125:629-640.

• György, Z., Jaakola, L., Neubauer, P. & Hohtola, A. (2009) Isolation and genotype-dependent, organ-specific expression analysis of a Rhodiola rosea cDNA encoding tyrosine decarboxylase. – Journal of Plant Physiology 166: 1581-1586.

• Jaakola, L., Riihinen, K., Häggman, H. & Hohtola, A.(2009) Bilberry, in vitro protocols and analyses of phenolic compounds. – In: Jain, S. Mohan; Saxena, Praveen K. (Eds.) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, pp. 71-80, Springer

• György, Z. & Hohtola, A. (2009) Production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea by biotransformation of cinnamyl alcohol. – In: Jain, S. Mohan; Saxena, Praveen K. (Eds.) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, pp. 305-312. Springer.

• Hohtola, A. (2009) Bioactive compounds from Northern plants. - In: Photosynthetic Organisms as Biological Farm for Production of Nutraceutics. M.T. Giardi, G. Rea & B. Berra (eds.): Biofarms for Nutraceuticals: Functional Food and Safety Control by Biosensors. Landes Bioscience and Springer Science+Business Media. Invited paper.

• Riihinen, K., Jaakola, L., Kärenlampi, S. & Hohtola, A.(2008) Organ-specific distribution of phenolic compounds in bilberry (Vaccinium myrtillus) and ‘northblue’ blueberry (Vaccinium corymbosum x V. angustifolium). – Food Chemistry 110: 156-160.

• Jaakola, L., Koskimäki, J. J., Riihinen, K.K., Tolvanen, A. & Hohtola, A. (2008) Effect of artificial wounding on chalcone synthase and pathogenesis related PR10 gene expression and content of phenolic compounds in bilberry leaves. - Biologia Plantarum 52:391-395.

• Pirttilä, A.M., Hohtola, A., Ivanova, E.G., Fedorov, D.N., Doronina, N.V. & Trotsenko, Y.A. (2008) Tissue specificity. Experimental procedures. Localization of plant-associated bacteria by in situ hybridization. - In: Sorvari, S. & Pirttilä, A.-M. (eds.) Prospects and Applications for Plant-Associated Microbes, pp. 61-64.

• Pirttilä, A.M., Hohtola, A., Ivanova, E.G., Fedorov, D.N., Doronina, N.V. & Trotsenko, Y.A. (2008) Tissue specificity. Theory. Identification and localization of methylotrophic plant-associated bacteria. - In: Sorvari, S. & Pirttilä, A.-M. (eds.) Prospects and Applications for Plant-Associated Microbes, pp. 218-224.

• Pirttilä, A.M., Podolich, O., Koskimäki, J. J., Hohtola, E. & Hohtola, A. (2008) Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.) – Plant, Cell, Tissue and Organ Culture 95: 47-55.

• Kämäräinen-Karppinen, T., Mäkinen, A., Kolehmainen, S., Hämäläinen, A., Laine, K., Hohtola, A., Mattila, S. & Pirttilä, A.M. (2008) Genetic and chemical variation in strains of clonally propagated French tarragon (Artemisia dracunculus var. sativa). - Journal of Horticultural Science and Biotechnology 83: 765-769.

• Pehkonen, T., Koskimäki, J., Riihinen, K., Pirttilä, A.M., Hohtola, A., Jaakola, L. & Tolvanen, A. (2008) Artificial infection of Vaccinium vitis-idaea L. and defence against Exobasidium fungal pathogens. - Physiological and Molecular Plant Pathology 72:146-150.

• Karppinen, K. Hokkanen, J., Mattila, S., Neubauer P. & Hohtola A. (2008) Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericins. FEBS Journal 275:4329-4342.

• Karppinen, K. & Hohtola, A. (2008) Molecular cloning and characterization of two cDNAs encoding polyketide synthases from Hypericum perforatum – Journal of Plant Physysiology 165:1079-1086.

• Jaakola, L., Koskimäki, J. J., Riihinen, K.K., Tolvanen, A. & Hohtola, A. (2008) Effect of artificial wounding on chalcone synthase and pathogenesis related PR10 gene expression and content of phenolic compounds in bilberry leaves. - Biologia Plantarum 391-395.

• Hohtola, A. (2007) Northern plants as a source of bioactive products. – In: "Physiology of Northern Plants at Changing Environment", editors Erja & Kari Taulavuori. Invited paper. Research Signpost.

• Pelkonen, V.-P. Niittyvuopio, A., Pirttilä, A. M., Laine, K. & Hohtola, A. (2007) Phylogenetic background of Orange lily (Lilium bulbiferum s.l.) cultivars from a genetically isolated environment. - Plant Biology 9: 534-540.

• Karppinen, K., Hokkanen, Juho, Tolonen, A., Mattila, S. & Hohtola, A. (2007) Biosynthesis of hyperforin and adhypeforin from amino acids precursors in shoot cultures of Hypericum perforatum. – Phytochemistry 68: 1038-1045.

• Karppinen, K., György, Z., Kauppinen, M., Tolonen, A., Jalonen, J., Neubauer, P, Hohtola, A. & Häggman, H, (2006) In vitro propagation of Hypericum perforatum L. and accumulation of hypericins, pseudohypericins and phloroglucinols. – Propagation of Ornamental Plants. 6: 170-179.

• Aflatuni A., Uusitalo, J., Ek, S. & Hohtola, A. (2006) Optimum harvesting time of four Mentha species in Northern Finland. – Journal of Essential Oil Research 18:134-138.

• Aflatuni, A., Uusitalo J., Ek, S. & Hohtola A. (2006) Variation in the amount of yield and in the extract composition between conventionally produced and micropropagated pepprmint and spearmint. J. Essent. Oil Res. 17:66-70.

• György, Z., Tolonen, A., Neubauer, P. & Hohtola, A. (2005) Enhanced biotransformation capacity of Rhodiola rosea callus cultures for glycoside production. Plant, Cell, Tissue and Organ Culture 83: 129-135.

• Pirttilä, A.M., Pospiech, H., Laukkanen H., Myllylä R. & Hohtola, A. (2005) Seasonal variation in location and population structure of endophytes in buds of Scots pine. Tree Physiol.25:289-297.

• Jaakola, L., Pirttilä, A.M. Vuosku, J. & Hohtola, A. (2004) A method based on electrophoresis and gel extraction for obtaining genomic DNA-free cDNA without DNase treatment. BioTechniques. 37:744-748.

• Vuosku, J., Jaakola, L., Jokipii, S., Karppinen, K., Kämäräinen, T., Pelkonen, V.-P., Jokela, A., Sarjala, T., Hohtola, A. & Häggman H. (2004) Does extraction of DNA and RNA by magnetic fishing work for diverse plant species? Mol. Biotechnol. 27:209-216.

• Pirttilä, A.M., Joensuu, P., Pospiech, H., Jalonen, J. & Hohtola, A. (2004) Bud endophytes of Scots pine produce adenine derivatives and other compounds that affect morphology and mitigate browning of callus cultures. Physiol. Plant. 121:305-312.

• Jaakola, L., Määttä-Riihinen, K., Kärenlampi, S. & Hohtola, A. (2004) Activation of flavonoid biosynthesis by solar radiation in bilberry (Vaccinium myrtillus L.) leaves. Planta 218:721-728.

• Tolonen, A., György, Z., Jalonen, J., Neubauer, P. & Hohtola, A. (2004) LC/MS/MS identification of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggrecates. Biomedical Chromatography. 18:550-558.

• György, Z., Tolonen, A., Pakonen, M., Neubauer P. & Hohtola A. (2003) Enhancing the production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea by biotransformation of cinnamyl alcohol. Plant Science 166:229-236.

• Aflatuni, A., Uusitalo, J., Ek, S. & Hohtola, A. (2003) Effect of liming on yield and quality of peppermint and Sachalin mint in fine sand soil of Northern Finland. Agr. Food Sci. Finland 12:107-115.

• Thoren, M., Tuomi, J., Kämäräinen, T. & Laine, K. (2003) Resource availability affects investment in carnivory in Drosera rotundifolia. New Phytologist 159: 507-511.

• Tolonen, A., Hohtola, A. & Jalonen, J. (2003) Liquid chromatochrapic analysis of phenylpropanoids from Rhodiola rosea extracts. Chromatographia 57:577-580.

• Tolonen, A., Pakonen M., Hohtola, A. & Jalonen, J. (2003) Phenylpropanoid glycosides from Rhodiola rosea. Chem. Pharm. Bull. 51:467-470.

• Kämäräinen, T., Uusitalo, J., Jalonen, J., Laine, K. & Hohtola, A. (2003) Regional and habitat differences in 7-methyljuglone content of Finnish Drosera rotundifolia. Phytochemistry 63: 309-314.

• Pirttilä, A. M., Laukkanen, H., Pospiech, H., Myllylä, R. & Hohtola, A. (2003) Two endophytic fungi in different tissues of Scots pine buds (Pinus sylvestris L.). Microbial Ecology 45:53-62.

• Tolonen, A., Hohtola, A. & Jalonen, J. (2003) Fast high performance liquid chromatographic analysis of naphtodianthrones and phloroglucinols from Hypericum perforatum extracts. Phytochemical Analysis 14: 306-309.

• Jaakola, L., Määttä, K., Pirttilä, A. M., Törrönen, R., Kärenlampi, S. & Hohtola, A. (2002) Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development. Plant Physiology 130:729-739.

• Tolonen, A., Uusitalo, J., Hohtola, A. & Jalonen, J. (2002) Determination of naphtodianthrones and phloroglucinols from Hypericum perforatum extracts by LC/MS/MS. Rapid Communications in Mass Spectrometry 16: 396-402.

• Tolonen, A., Joutsamo, T., Mattila, S., Kämäräinen, T & Jalonen, J. (2002) Identification of isomeric dicaffeoylquinic acids from Eleutherococcus senticosus using HPLC-ESI/TOF/MS and 1H-NMR methods. Phytochemical analysis 13: 316-328.

• Hirsikorpi, M., Kämäräinen, T., Teeri, T., Hohtola, A. (2002) Agrobacterium tumefaciens - mediated transformation of round leaved sundew (Drosera rotundifolia L.). Plant Science 162:537-542.

• Pirttilä, A. M., Laukkanen, H. & Hohtola A. (2002) Chitinase production in pine callus (Pinus sylvestris L.): a defence reaction against endophytes? Planta 214: 848-852.

• Pirttilä, AM., Hirsikorpi M., Kämäräinen, T., Jaakola, L. & Hohtola, A. (2001) DNA isolation methods for medicinal and aromatic plants. Plant Mol. Biol. Reptr. 19: 273a-f.

• Jaakola, L., Pirttilä, AM. & Hohtola, A. (2001) cDNA blotting offers an alternative method for gene expression studies. Plant Mol. Biol. Reptr. 19:125-128.

• Jaakola, L., Tolvanen A., Laine, K. & Hohtola, A. (2001) Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots. Plant Cell Tiss. Org. Cult. 66: 73-77.

• Jaakola, L., Pirttilä, A. M., Halonen, M. & Hohtola, A. (2001) Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Mol. Biotechnol. 19: 201-203.

  
  
  
  

  updated 30.11.2009

    

  Laura.Jaakola(at)oulu.fi